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Urogenital infections
JŮZOVÁ, Zdeňka
Bachelor thesis deals with infections affecting the urogenital tract of women in fertile age. The theoretical part is focused on all producers of urogenital infections causing complications for women, which include the total discomfort as in sex, difficulty in urination or postinflammatory complications for example infertility, but also discusses the adverse effects of infections on pregnancies and newborns. The practical part was conducted with professional assistance of staff in the microbiology laboratory synlab czech s.r.o in České Budějovice. There I learned to process sampled biological material (vaginal / cervical swab, urine), types of used agars to cultivation (UriSelect, KA, Sabouraud agar, GAR and MHA), various identification methods to confirm the microbe predetermined Dr. microbiologist according to macroscopic features and preparation with Gram-staining, among them Clumping factor, latex agglutination, PYR - test CAMP - test, IBK and instrument identification by MALDI - TOF. Then I made the cultivation for the presence of Mycoplasma hominis, Ureaplasma urealyticum and testing for the presence of antigen Chlamydia trachomatis by ELISA. And finally I performed the antimicrobial susceptibility testing with disk diffusion method. From processing of the results I found that among 148 women over a period of 1 to 18 September 2015 were most often found in samples of bacterial agens, particularly coagulase - negative Staphylococci, E. coli and Streptococcus agalactiae. I didn't find of Chlamydia trachomatis in any sample the selected group of women, Mycoplasma hominis was isolated only from one sample and Ureaplasma urealyticum was among five women.
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Investigation of chlamydia urogenital tract
BENDOVÁ, Radka
The subject of this bachelor paper is chlamydiae examination in urogenital tract via the method of cultivation. The aim of this paper is to test the cultivation method of diagnosis Chlamydia trachomatis on a tissue of culture, to evaluate the outcome according to individual criteria in a given period and to compare the outcome with the literary data. The virology laboratory in hospital in České Budějovice, a.s. Is equiped for the culture tissue preparation and for this method they use uncommon line of cells BGM, which is not commonly used in the world. In this department this line has proven and so they use it since 1985, when they received it. This paper informs about the present situation of chlamydiae, their clasification according to phylogenetic and genome bacterial analysis, about their occurence, growth cycle and multiplication. The paper also focuses on chlamydiae infections divided into ocular and urogenital infection and their treatment. The overview of present issues is finished by the methods of evidence, direct and indirect evidence. In the direct evidence there are described the issue cultures their preparation and cultivation. This method was verified in the practical part of my bachelor paper. For this bachelor paper I was provided with samples from urogenital tract which were evaluated in three categories: positive, negative and imposible to evaluate (samples were overgrown with bacteria or yeast cells and it was imposible to evaluate them). They were mostly smears from urethra and cervix (at women) or from urethra only (at men). The number of all samples was 159 and they were examined during the period from 18th October to 5th December 2011. Chlamydiae grew on issue cultures for 48 hours and then they were died with modified colouring Macchiavella. 127 examples (79.9%) coloured by this technique were evaluated from the total number. Ambiguous samples were coloured by the monoclonal antidote against Chlamydia trachomatis. 32 samples (20.1%) were coloured using this technique from the total number.I also evaluated the samples according to age. Unusually high was positivity (42.2%) at women at the age of 26-46. Which includes the highest number of samples (54.2%) of women from the total number. It mostly concerned pacients with gynaecological difficulties who were never examined and treated or they had a chronical infection which can be treated with difficulties and inclinates to relapse. At men the highest number of samples (56.1%) from the total number was at the age of 26-46 but only 13% were positive. The difference is given by the size of samples and also at men the occurence of chronical infection is less common.
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MIDWIFE´S ROLE IN EDUCATION OF WOMEN WITH PELVIC INFLAMMATORY DISEASE
VLČKOVÁ, Hana
MIDWIFE´S ROLE IN EDUCATION OF WOMEN WITH PELVIC INFLAMMATORY DISEASE Nowadays, the number of women who have underestimated the symptoms of pelvic inflammatory disease and who have been coping with its consequences such as chronic pain or infertility is increasing. Therefore the following topic was selected for the bachelor thesis: "Midwife´s Role in Education of Women with Pelvic Inflammatory Disease". The theoretical part of the bachelor thesis deals with the educational activity provided by a midwife, basic aetiology of pelvic inflammatory disease, its treatment and nursing process for women with pelvic inflammatory disease. A quantitative research method using anonymous questionnaires was selected for the practical part of the bachelor thesis. The research was conducted in the period of 1 January - 31 March 2011 and 93 responders participated in it. Three goals were stated in the thesis and they were met. The first goal of the research was to find out whether women are informed about the symptoms, treatment and subsequent prevention of pelvic inflammatory disease. The second goal was to find out how satisfied women are with the level of education provided by a midwife in the field of pelvic inflammatory disease. The third goal was to create educational material on pelvic inflammatory disease. Further, for the purpose of the research, 3 hypotheses were stated. Hypothesis No. 1: "Women are aware of the symptoms of pelvic inflammatory disease and they pay respective attention to them," was confirmed. Hypothesis No. 2: "Women lack information on causative agents of pelvic inflammatory disease causing post-inflammatory changes such as infertility," was confirmed. Hypothesis No. 3: !Women have enough information on causes of occurrence of gynaecological inflammation, its symptoms, treatment and subsequent prevention," was not confirmed. The results of the research investigation of this thesis could be useful for midwives and their educational activity. Educational material in form of a leaflet was created for women´s needs.
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